Figure 3.

CD29-induced pro-survival signalling depends on ROS. (A–C) NRVM were infected with adenoviral constructs to express β-galactosidase (LacZ₁₀₀) at 100 MOI or co-overexpress SOD (S₅₀) and cat (C₅₀) at 50 MOI 36 h prior to α-CD29 administration (10 µg/mL, 15 min). Equal protein amounts were subjected to western blotting using phosphorylation-specific antibodies for MEK1/2 (Ser217/221), Akt (Ser473), and GSK-3β (Ser9). Data are presented as mean ± SEM. *** and ^###P< 0.001, ** and ^##P< 0.01, and ^#P< 0.05 (n= 5 for A and B, n= 3 for C). Unless indicated otherwise, comparisons were not significant. (D) Western blot of CD29-induced signalling with single-enzyme overexpression at two different MOI, including the protein expressions of SOD and cat.