Figure 4.

CD29-induced signal transduction requires NOX2-derived ROS. α-CD29 administration (10 µg/mL, 15 min) induced phosphorylation of ERK1/2 (p44/42), Akt and GSK-3β in wild-type (wt) NMVM (A–F). α-CD29-induced phosphorylation was absent in NOX2 knock-out (NOX2^k.o.) cardiomyocytes (A–C); and partially diminished in p47^phox loss of function (p47^LOF) NMVM (D–F). Data are presented as mean ± SEM. Unless indicated otherwise, comparisons were not significant. * and ^#P< 0.05, ** and ^##P< 0.01, *** and ^###P< 0.001 (n= 5 for A, C, and D–F; n= 6 for B).