Table 1.
Platelet function assay (PFA)
| Method of PFA | High platelet reactivity | Comments |
|---|---|---|
| LTA (5 micro mol/L ADP) | >42.9% aggregation | Requires dedicated lab with trained technicians; labor and time intensive |
| LTA (20 micro mol/L ADP) | >64.5% aggregation | Requires dedicated lab with trained technicians; labor and time intensive |
| VerifyNow P2Y12 assay | >236 PRU | Fully automated and POC and turnaround time for test is 15 minutes. VerifyNow P2Y12 Assay (Accumetrics, San Diego, California) |
| VASP-P | >50 PRI | Requires a dedicated lab. Measures platelet reactivity index. Requires a flow cytometer |
| Plateletworks assay | >80.5% aggregation | Semi-automated POC, time dependent; Helena Laboratories, Beaumont, Texas |
| PFA 100 (Collagen/ADP cartridge) | Closure time <116 seconds | POC; Siemens Healthcare Diagnostic GMBH, Marburg Germany |
| IMPACT-R Assay | >8.4% surface coverage | Extensive sample handing; Matis Medical Inc, |
| With and without ADP | >3.0% surface coverage | Beersel, Belgium |
| Innovance PFA P2Y | >299 seconds | POC; Siemens Healthcare Diagnostic GMBH, Marburg Germany |
Abbreviations: ADP, adenosine diphosphate; LTA, light transmittance aggregometry; POC, point of care; PRI, platelet reactivity index; PRU, P2Y12 reaction unit; VASP-P, vasodilator-stimulated phosphoprotein phosphorylation.