Table 3. Kinetic parameters of wild-type and D204N mutant AdoP with adenosine and inosine as substrates.

	Km (µM)	kcat (s−1)	kcat/Km (s−1 M−1)
Wild type
Adenosine	23 ± 6	75 ± 5	3.1 × 106
Inosine	315 ± 52	9.3 ± 0.5	3.0 × 104
D204N
Adenosine	24 ± 7	0.18 ± 0.01	7.3 × 103
Inosine	102 ± 11	6.8 ± 0.2	6.6 × 104