Table 2.
RNA purification from tomato and Arabidopsis leaves, petunia flowers and Botrytis cinerea mycelium by LogSpin protocol and by commercial kits
RNA yield1 | Absorbance | ||||
---|---|---|---|---|---|
Organism | Protocol | μg/100 mg | 260/280 | 260/230 | |
1 | Tomato | TRI2 | 31.3 ± 6.2 | 2.07 ± 0.01 | 2.27 ± 0.01 |
3 | Tomato | RNeasy3 | 26.7 ± 13.3 | 2.11 ± 0.00 | 2.33 ± 0.04 |
2 | Tomato | LogSpin | 25.1 ± 1.8 | 2.14 ± 0.01 | 2.30 ± 0.01 |
4 | Arabidopsis | TRI1 | 21.1 ± 5.9 | 2.13 ± 0.00 | 2.05 ± 0.12 |
5 | Arabidopsis | RNeasy3 | 16.3 ± 3.8 | 2.10 ± 0.01 | 2.28 ± 0.01 |
6 | Arabidopsis | LogSpin | 15.5 ± 3.6 | 2.15 ± 0.01 | 2.29 ± 0.01 |
7 | Petunia | TRI2 | 37.8 ± 5.5 | 1.90 ± 0.06 | 1.30 ± 0.25 |
8 | Petunia | RNeasy3 | 14.7 ± 1.0 | 2.07 ± 0.02 | 2.02 ± 0.14 |
9 | Petunia | LogSpin | 13.6 ± 0.5 | 2.06 ± 0.02 | 2.21 ± 0.01 |
10 | B. cinerea | KIT4 | 31.0 ± 3.4 | 2.12 ± 0.00 | 2.14 ± 0.00 |
11 | B. cinerea | LogSpin | 83.3 ± 18.9 | 1.87 ± 0.07 | 2.05 ± 0.08 |
1RNA yield ± SE (n = 3-10).
2Tri-reagent, Sigma-Aldrich.
3RNeasy, Qiagen.
4Norgen.