Skip to main content
. 2012 Jan 19;5:45. doi: 10.1186/1756-0500-5-45

Table 2.

RNA purification from tomato and Arabidopsis leaves, petunia flowers and Botrytis cinerea mycelium by LogSpin protocol and by commercial kits

RNA yield1 Absorbance
Organism Protocol μg/100 mg 260/280 260/230
1 Tomato TRI2 31.3 ± 6.2 2.07 ± 0.01 2.27 ± 0.01

3 Tomato RNeasy3 26.7 ± 13.3 2.11 ± 0.00 2.33 ± 0.04

2 Tomato LogSpin 25.1 ± 1.8 2.14 ± 0.01 2.30 ± 0.01

4 Arabidopsis TRI1 21.1 ± 5.9 2.13 ± 0.00 2.05 ± 0.12

5 Arabidopsis RNeasy3 16.3 ± 3.8 2.10 ± 0.01 2.28 ± 0.01

6 Arabidopsis LogSpin 15.5 ± 3.6 2.15 ± 0.01 2.29 ± 0.01

7 Petunia TRI2 37.8 ± 5.5 1.90 ± 0.06 1.30 ± 0.25

8 Petunia RNeasy3 14.7 ± 1.0 2.07 ± 0.02 2.02 ± 0.14

9 Petunia LogSpin 13.6 ± 0.5 2.06 ± 0.02 2.21 ± 0.01

10 B. cinerea KIT4 31.0 ± 3.4 2.12 ± 0.00 2.14 ± 0.00

11 B. cinerea LogSpin 83.3 ± 18.9 1.87 ± 0.07 2.05 ± 0.08

1RNA yield ± SE (n = 3-10).

2Tri-reagent, Sigma-Aldrich.

3RNeasy, Qiagen.

4Norgen.