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Cell morphology and cell viability after NGF induction. PC12 cells differentiate with neurite extension after NGF induction for 8 days (A-a). Some cell debris (arrowheads) and degenerating neurites are observed in pEGFP-Peripheirn cells after 8 days of NGF induction (A-b). (B) Cell viability assessed by Trypan blue exclusion on different days of NGF induction. * *p < 0.01, *** p < 0.001 in the t-test; n = 4. Scale bar = 20 μm.
