Table 2.
Blastx analyses of SDIMO clone sequences representing each of the OPF determined in the present study and the highest identity matches from the Genbank database.
| OPF | Number of clone sequences | Gene | Best match | Average of Identity (nt) |
|---|---|---|---|---|
| 1 | 5 (P) - 14 (Np) | SDIMO | Uncultured bacterium ABB70434 | 81.4% |
| 2 | 48 (P) - 56 (Np) | SDIMO | Uncultured bacterium ABB70441 | 93.2% |
| 3 | 3 (P) | SDIMO | Uncultured bacterium ABB70469 | 93.4% |
| 4 | 3 (P) | EtnC | Mycobacterium chubuense NBB4 | 83.7% |
| 5 | 1 (P) | SDIMO | Mycobacterium sp. JS623 | 66.0% |
| 6 | 6 (P) | SDIMO | Uncultured bacterium ABB70430 | 77.4% |
| 7 | 2 (P) | MmoX | Nocardioides sp. JS614 | 88.5% |
MmoX represents Methane Monoxygenase, EtnC represents Ethene Monoxygenase, and SDIMOs represents soluble di-iron monoxygenase enzymes that have not been assigned to a known subgroup. Identity average shows the percentage of identity at the nucleotide level (nt) compared with sequences from Genbank database. (P) Represents sequences from P soil and (Np) from Np soil.