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. 2011 Dec 16;12:65. doi: 10.1186/1471-2091-12-65

Table 5.

Sense primers for hNPP7ex-FLAG mutations.

NPP7 mutation Sense primer
F80A TGACCAGCC CTGCCACGCC ACCCTGGTCA CCGGCAAATA

L107F ACCACCAGCA AGGTGAAG TTC CCCTACCACGCCACGCTGG

Y142A TGGCTCCTTC TTCGCCCCGG GCGGGAACGT

Y166A GGCATCGCAC ACAACGCCAAAAATGAGACG

E169A TCGCAC ACAACTACAAAAATGCGACG GAGTGGAGAG

E169Q TCGCAC ACAACTACAAAAATGAGACG GAGTGGAGAG

Y194A ATCTGGTCACACTCGCCTTCGGGGAGCCGGACTCC

F275A CGGGACATCG AGTTTGAGCT CCTGGACTAC