Figure 6. Gel filtered Klp6₄₄₀ promotes microtubule nucleation.

A fraction from the single protein elution peak of Klp6₄₄₀His gel filtered on a Superose 12 column (A) was immediately incubated with 1 µM S. pombe tubulin in buffer containing 1 mM GMPCPP at 25°C. (B) Aliquots of samples containing 0, 5 and 28 nM Klp6₄₄₀His were analysed by darkfield microscopy (Scale bar: 10 µm). Microtubule lengths and numbers were measured and plotted against Klp6₄₄₀His concentration (C). Increasing Klp6₄₄₀His concentration caused an increase in microtubule number and decrease in microtubule length.