Table 1. Microtubule gliding assays.
Pig brain taxol MTs (nm s−1) | S. pombe GMPCPP MTs (nm s−1) | Pig brain GMPCPP MTs (nm s−1) | |
Klp6440His | 87±18 (1085) | 23±12 (77) | 56±19 (178) |
Klp5436GST | 6.6±3.7 (311) | 6.5±3.6 (115) | Not determined |
Microtubules were assembled from pig brain tubulin and stabilised with Taxol or S. pombe tubulin stabilised with GMPCPP. The velocity of microtubule gliding over surfaces coated with either Klp6 or Klp5 was determined at 25°C. Velocities are mean ± Standard deviation (n).