Table 2. Microtubule and tubulin stimulated Klp5/6 ATPase activity.
| Microtubules | Tubulin heterodimer | ||||
| Vmax (s−1) | Km (nM) | Vmax (s−1) | Km (nM) | ||
| Pig brain | Klp5436GST | 2.3±0.1 | 27.8±4.9 | 0.36±0.03 | 88±26 |
| Klp5436 | 3.1±0.2 | 45±11 | 0.36±0.04 | 427±120 | |
| Klp6440His | 6.3±0.6 | 85±26 | 0.032±0.004 | 337±123 | |
| S. pombe | Klp5436GST | 3.4±0.2 | 45±11 | 0.49±0.04 | 108±40 |
| Klp5436 | 5.0±0.1 | 50.0±4.6 | 0.6±0.1 | 336±145 | |
| Klp6440His | 2.5±0.2 | 56±14 | 0.097±0.009 | 373±87 | |
Microtubules were assembled from either pig brain tubulin and stabilised with Taxol or S. pombe tubulin stabilised with GMPCPP. Microtubule or tubulin heterodimer stimulated ATPase activities were determined in linked assays at 25°C. Non-linear fits of plots of ATPase activity against tubulin concentration were used to determine Vmax and Km values ± standard error of mean.