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. 2012 Feb 20;7(2):e31616. doi: 10.1371/journal.pone.0031616

Figure 3. Generation of Apolipoprotein F Deficient Mice.

Figure 3

A. Schematic diagram (not to scale) showing the wild type and deleted ApoF alleles. Features are depicted as follows: Exons- white arrows, Beta galactosidase reporter gene- “LacZ” black arrow, PGK promoter- grey arrow, Neomycin resistance gene- “Neo” black arrow, BamHI restriction sites- vertical lines, and the location of Southern Blotting probe- asterisk. B. Southern blot confirming successful targeting and genomic location of null allele: WT allele yields a 3.9 kb band, while the Null allele is 2.4 kb. The left panel contains ES cell DNA, while the right panel depicts livers from the mice. C. Real Time RT-PCR data on ApoF mRNA in the livers of female wild type (+/+), heterozygous (+/−), and homozygous (−/−) ApoF deficient mice.