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. 2012 Feb 20;7(2):e31616. doi: 10.1371/journal.pone.0031616

Figure 5. HDL cholesterol content, size, and apoprotein composition in ApoF KO mice.

Figure 5

A. Gel filtration chromatography of 150 microliters of pooled plasma from wild type (WT), and ApoF KO (KO) mice. B. HDL size of plasma from wild type (WT), heterozygous (Het) and homozygous ApoF KO (KO) mice as determined by NMR analysis. C. Denaturing SDS-PAGE gel of proteins from HDL (1.063<d<1.21) centrifugally isolated from wild type and ApoF knockout mice. Ten micrograms of HDL protein was electrophoresed on 12% Bis-Tris gels and subjected to staining with Coomassie blue. The identity of albumin, apo E, apo A-I, apo A-II, and apo C's were determined by comparison to standards of human LDL, HDL and whole plasma. The 13 kDa band present in the wild type HDL but diminished in the KO was found to contain Apo N and B-globin as the primary protein components by LC-MS.