Table 3. The effects of High fat feeding on plasma lipids in ApoF deficient mice.

	Genotype	Cholesterol (mg /dl)	HDL-C (mg/dl)	Non HDL-C (mg/dl)	Triglycerides (mg/dl)	Cholesteryl Ester (mg/dl)	Free Cholesterol (mg/dl)
	WT	79+/−6	53+/−5	26+/−2	25+/−6	50+/−5	29+/−6
Baseline	Het	79+/−7	54+/−6	25+/−2	18+/−6	54 +/−6	26+/−5
	KO	76+/−10	53+/−7	24+/−5	23+/−7	53+/−8	23+/−2*
	WT	126+/−8	95+/−6	31+/−5	25+/−27	89+/−10	37+/−8
2 weeks	Het	120+/−14	93+/−8	27+/−12	12+/−4*	81+/−13	40+/−7
	KO	121+/−11	92+/−10	29+/−3	11+/−1*	90+/−10	32+/−3
	WT	125+/−9	92+/−8	34+/−3	31+/−7	82+/−10	43+/−4
4 weeks	Het	122+/−10	90+/−7	33+/−5	16+/−4**	84+/−7	38+/−5
	KO	116+/−7	84+/−6	32+/−3	16+/−6**	79+/−6	37+/−2
	WT	131+/−8	98+/−7	33+/−5	21+/−8	82+/−8	48+/−3
9 weeks	Het	132+/−9	97+/−9	35+/−4	21+/−4	86+/−7	47+/−3
	KO	124+/−7	88+/−6	36+/−3	20+/−5	78+/−6	46+/−6

Female ApoF wild type (WT), heterozygous (Het), and knockout (KO) mice were bled for baseline lipid measurements and placed on a diet containing 45% kcal from fat for 9 weeks. Plasma was then collected after 2, 4, and 9 weeks on the diet. All mice were fasted 4 hours before bleeding at every time point. Values are reported as the mean +/− standard deviation for each group (WT n = 8, Het n = 10, KO n = 8).

Two Way ANOVA Results:

Cholesterol: Time p<0.0001.

HDL-C: Time p<0.0001.

Non HDL-C: Time p<0.0001.

Triglycerides: Time n.s.,Genotype p = 0.0025 (Bonferroni vs WT: * p<0.05, ** p<0.01).

CE: Interaction p = 0.0428, Time p<0.0001.

Free Chol: Time p<0.0001, Genotype p = 0.0003 (Bonferroni WT vs KO *p<0.05).