Table 5. Lipoprotein Composition in ApoF KO mice.
Fraction | Genotype | Protein | Cholesteryl Ester | Free Cholesterol | Triglycerides | Phospholipids |
VLDL | WT | 37.4+/−4.8 | 1.6+/−0.3 | 1.6+/−0.1 | 55.2+/−5.3 | 4.3+/−1.3 |
KO | 44.3+/−6.2 | 1.4+/−0.1 | 1.3+/−0.4 | 49.3+/−6.7 | 3.7+/−1.2 | |
IDL/LDL | WT | 21.8+/−1.2 | 8.3+/−2.0 | 9.3+/−0.4 | 45.8+/−4.0 | 14.7+/−1.4 |
KO | 24.6+/−3.1 | 8.5+/−2.2 | 10.5+/−1.6 | 38.6+/−4.3 | 17.8+/−10 | |
HDL | WT | 48.6+/−0.9 | 22.7+/−1.1 | 5.6+/−0.2 | 0.62+/−0.06 | 22.4+/−0.4 |
KO | 47.7+/−1.1 | 20.8+/−2.4 | 5.9+/−0.8 | 0.72+/−0.21 | 24.9+/−2.7 |
Lipoproteins were isolated from one milliliter of pooled mouse plasma by KBr density gradient ultracentrifugation as follows: VLDL d<1.006, IDL/LDL 1.006<d<1.063, HDL 1.063<d<1.21 g/mL. Concentrations for each lipid are given as the percentage of total mass in the fraction. Values are reported as the mean +/− standard deviation of three separate isolations (n = 3), with each value representing a separate and distinct pool of at least four mice per genotype.