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. 2012 Feb 20;7(2):e31573. doi: 10.1371/journal.pone.0031573

Figure 2. Dependence of HDAC-inhibitory activity for t-PA induction and effect of VPA on global histone acetylation.

Figure 2

A. t-PA mRNA expression in HUVECs stimulated with 4 mM VPA or 4 mM VPM (a structural amide analogue of VPA which is reported to lack HDAC inhibitory activity) for 24 h. The results are presented as mean values ± SEM of five independent experiments performed in duplicate. **p<0.01. B. HUVECs were exposed to 3 mM VPA for 24 h after which cells were harvested in Laemmli sample buffer for analysis of global acetylation of histone H3 and H4 by Western blotting. Data are representative of three independent experiments.