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. 2012 Feb 20;7(2):e31573. doi: 10.1371/journal.pone.0031573

Figure 3. Chromatin Immunoprecipitation (ChIP) for acetylated histones in the t-PA promoter.

Figure 3

HUVECs were exposed to 3 mM of VPA for 24 h after which cells were fixed and chromatin harvested. ChIP-analyses for acetylated histone H3 and H4 were performed with real-time PCR primers flanking the major t-PA transcription start site. Data are presented as percent input corrected for background binding and mean values ± SEM of four to five independent experiments are shown. A. ChIP for pan-acetylated histone H3. n = 4. B. ChIP for pan-acetylated H4. n = 5. C. ChIP for specific histone H3 acetylation. Antibodies for monoacetylated acH3K9, acH3K18, acH3K23 and acH3K27 were used. n = 4. D. ChIP for specific histone H4 acetylation. Antibodies for monoacetylated acH4K5, acH4K8, acH4K12 and acH4K16 were used. n = 5. *p<0.05, **p<0.01, *** p<0.001.