Figure 4. siRNA mediated knock-down of class I HDACs.

t-PA mRNA expression in HUVECs treated with class I HDAC-targeting siRNAs for 72 h and then stimulated with 3 mM VPA for 24 h. Mock indicate cells treated with DharmaFECT 4 transfection reagent alone and ASN indicate cells transfected with All Star Negative control siRNA (Qiagen). Unless indicated in the figure, statistical comparisons are made relative to untreated control cells. *p<0.05, **p<0.01. The results are presented as mean values ± SEM of four independent experiments performed in duplicate.