Skip to main content
. 2012 Feb 20;7(2):e31573. doi: 10.1371/journal.pone.0031573

Figure 5. siRNA mediated knock-down of class IIa and class IV HDACs.

Figure 5

t-PA mRNA expression in HUVECs treated with A. HDAC4, B. HDAC5, C. HDAC7, D. HDAC9 and E. HDAC11-targeting siRNA for 72 h and then stimulated with 3 mM VPA for 24 h. Mock indicate cells treated with DharmaFECT 4 transfection reagent alone and ASN indicate cells transfected with All Star Negative control siRNA (Qiagen). Unless indicated in the figure, statistical comparisons are made relative to untreated control cells. *p<0.05, **p<0.01. The results are presented as mean values ± SEM of four independent experiments performed in duplicate (HDAC9, n = 2).