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. 2012 Feb 20;7(2):e31765. doi: 10.1371/journal.pone.0031765

Figure 2. Negative regulation of motility by the Gac system acts through downregulation of the fleQ gene transcription during exponential phase.

Figure 2

(A) RT-PCR expression analysis of fliC (primers fliCF-R), fleQ (primers fleQF-R), and 16S (primers 16SF-R) genes of F113 (1), gacA − (2), gacS − (3), F113 prsmA (4), and F113 prsmE (5). (B) Western blot analysis of external proteins from F113 (1), gacA − (2), gacS − (3), F113 prsmA (4), and F113 prsmE (5) during exponential phase (O.D.600 = 0.3) (a), and stationary phase (O.D.600 = 3.5) (b), reacted with an anti-flagellin antiserum. The observed band is approximately 35 KDa and corresponds to FliC. (C) Percentage of flagellated cells of F113 wild-type (black bar) or gacS − (grey bar) during exponential phase (O.D.600 = 0.3) (a), and stationary phase (O.D.600 = 3.5) (b). Statistical significance is shown.