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. 2012 Feb 20;7(2):e31765. doi: 10.1371/journal.pone.0031765

Figure 5. SadB and GacAS regulate algU expression.

Figure 5

(A) Analysis of the swimming motility of F113 wild-type, mutants in the Gac-SadB-AlgU cascade, and amrZ complementation (pamrZ). Different letters indicate significant statistical differences (p<0.05). At 18 h, F113 wild-type strain swimming halo diameter is 11±0.55 (mm). (B) qRT-PCR expression analysis of algU (black) and amrZ (grey) genes (primers qalgUF-R and qamrZF-R, respectively) in F113 wild-type, gacS −, sadB −, and double mutant gacS-sadB. 16S gene expression (primers 16SF-R) was used for normalization. Different letters indicate significant statistical differences (p<0.05).