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. 2012 Feb 20;7(2):e31737. doi: 10.1371/journal.pone.0031737

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Lillvis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Backfilling the pedal commissure with biocytin labeled 3–4 neurons near C2_Mel (left). Only the cerebral-pleural ganglion contralateral to the backfilled nerve is shown. FMRFamide-like immunohistochemistry labeled C2_Mel (middle). Combining FMRFamide-like immunoreactivity with the backfill revealed just one neuron, C2_Mel (right). B. C2_Mel could be identified visually due to its characteristic white soma near the origin of cerebral nerve 1 (CeN1, white arrows). An asterisk (*) labels the statocyst. Only one cerebral-pleural ganglion is shown. C. Filling the C2_Mel soma with Neurobiotin revealed a branching contralateral axon projection through the anterior cerebral-pedal commissure (not shown) and into the pedal commissure (PP2). The example is a representative image in which the outline of the brain and the axon projection were traced for ease of viewing. D. C2_Mel (arrow) was filled with biocytin (left). It was immunoreactive for both FMRFamide (middle) and SCP_B (right) as shown.