Figure 1. In vitro hyperthermia differentially regulates the expression of maturation markers on BMDCs.

Immature or LPS (1 µg/ml; 6 hours) matured BMDCs were either maintained at 37°C or exposed to hyperthermia for 42°C for 30 minutes and analyzed by flow cytometry at indicated recovery periods, after staining with anti-CD80, anti-CD86, anti-MHC-II, anti-CD40, anti-CCR5 and anti-CCR7 mAbs. Results are shown as dot plots indicating the percentage of cells expressing the various markers and are representative of three independent experiments.