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. 2012 Feb 20;7(2):e32067. doi: 10.1371/journal.pone.0032067

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Joshi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mice were immunized by TT, with and without HET. Unimmunized (PBS without HET) animals served as control. Cell suspension from draining and non-draining lymph nodes were prepared after 24 h and stained with Langerin (CD207)-PE and EpCAM-Alexa Flour 488 antibodies. Stained cells were analyzed by flow cytometry, Langerin+ cells were gated and the expression of EpCAM was analyzed. Number on the selected area indicates percent of EpCAM+ cells among Langerin+ cells in total lymph node cells. The bar graph shows average percentages of three experiments.