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. 2012 Feb 20;7(2):e32067. doi: 10.1371/journal.pone.0032067

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Joshi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mice were immunized by soluble TT alone (i/m), TT with alum (i/m) or TT via HET. Unimmunized animal served as control. Booster of soluble TT (i/m) was given on Day 28 and mice were bled on week 5. Serum anti TT IgG1 (A1), IgG2a (A2) and IgG (A3) were estimated by ELISA. Recall response was evaluated by estimating the proliferative index by in vitro restimulation of splenocytes from immunized animals with TT antigen (B). Each dot represents individual animal and bars represent the geometric mean of each group.