Figure 3. Quantification of vestibular hair cell orientation in control and vangl2 mutant utricles.

(A) Circular histograms demonstrating the orientations of all of the vestibular hair cells that were measured from fields1,2&3 for each of the genotypes listed. The number of cells in each bin is graphed along the x-axis and the cell total is listed. The average orientation of each group of cells is marked by a bold black line. (B) The averaged mean deviation of stereocilia bundle orientations from 0° was determined by measuring the absolute value of the angle formed by the bundle axis and a reference line drawn perpendicular to the striola. In this schematic red indicates the position of the kinocilium and green is the cell periphery. (C) The averaged deviation from 0° for hair cells located in fields1,2&3 for each experimental and control genotype. Error bars indicate standard deviation. Statistical significance was calculated by Student's t-test with unequal variance. (*P<0.05, **P<0.001, ***P<10⁻⁵).