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. 2012 Feb 20;7(2):e32019. doi: 10.1371/journal.pone.0032019

Figure 2. Effect of clustered ephrin-A1-Fc injection in rats with chemical lesions in the unilateral nigrostriatal dopaminergic pathway.

Figure 2

(A) Effect of intrastriatal injection of clustered ephrin-A1-Fc on the regeneration of dopaminergic cells. Rats with unilateral nigrostriatal lesions were injected with clustered IgG(Fc) (upper panels) or ephrin-A1-Fc (lower panels) into the lesioned side of the striatum near the subventricular zone. Rats were killed 6 weeks after injection. Brains were sectioned coronally, and sections were stained immunohistochemically for tyrosine hydroxylase (TH). Yellow arrows indicate the course of needles used for injection of clustered IgG(Fc) or ephrin-A1-Fc. Scale bar: 100 µm. (B) Entire striatal coronal view of TH–positive cells. Clustered IgG(Fc) or ephrin-A1-Fc was injected into the ventricle ipsilateral to the lesioned side, and coronal sections of the entire striatum were stained for TH. Upper panels, clustered IgG(Fc)-injected rat; lower panels, clustered ephrin-A1-Fc-injected rat. Scale bar: 500 µm.