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. 2012 Feb 13;61(3):653–658. doi: 10.2337/db11-0944

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© 2012 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 1. — miR-33a modulates ABCA1 expression in pancreatic islets. A: Quantitative PCR analysis of miR-33a expression in human and wild-type mouse islets and liver and MIN6 mouse insulinoma cell line. B: Immunostaining for ABCA1 (red) in fixed and permeabilized wild-type mouse islets infected with LacZ, miR-33a, or ABCA1 adenovirus (Ad). C: ABCA1 expression as assessed by Western blot in wild-type mouse islets infected with recombinant Ad-expressing GFP, miR-33a (n = 3), or miR-33a-Inh (n = 3). Quantified values are normalized to β-tubulin. *P < 0.05, **P < 0.01. (A high-quality digital representation of this figure is available in the online issue.)