FIG. 1.

Diabetic renal injury was accelerated in diabetic CKO mice. At 4 and 10 weeks after the induction of diabetes, parameters of DN were analyzed. A and B: Urinary protein excretion was measured by Bradford assay (A) or SDS-PAGE (B). C: Plasma creatinine was measured by Detect X Serum Creatinine Detection Kit. D–F: The kidneys were fixed, cut into 4-μm sections, and stained with PAS reagent (D). Scale bar, 20 μm; magnification ×630. After PAS staining, the glomerular volume (E) and FMA (F) were analyzed using Image Pro Plus (50 glomeruli per animal). WT, nondiabetic control mice; WTDM, STZ-induced diabetic WT mice; CKODM, STZ-induced diabetic CKO mice. Data are mean ± SE of n = 7–8 mice per group. *P < 0.05 vs. WT; †P < 0.05 vs. WTDM at the same duration of diabetes; **P < 0.05 between two groups.