FIG. 3.

ECM expression was further increased in diabetic CKO kidneys. A and B: Renal FN mRNA (A) and protein (B) expressions at 4 and 10 weeks after the induction of diabetes. C: The renal tissues of 4 weeks of diabetes were immunostained with anti–FN ED-A (1:200; left), and FN ED-A positive area was quantified by using ImageJ software (right; 15 pictures per animal). Single scale bar, 100 μm; magnification ×200. Double scale bar, 20 μm; magnification ×630. D: Renal collagen was measured by Masson trichrome staining at 10 weeks after the induction of diabetes (left) and quantified (right; 15 pictures per animal). The upper and lower pictures are magnification ×12.5 (scale bar, 1 mm) and ×630 (scale bar, 20 μm), respectively. WT, nondiabetic control mice; WTDM, STZ-induced diabetic WT mice; CKODM, STZ-induced diabetic CKO mice. Data are mean ± SE of n = 7–8 mice per group. *P < 0.05 vs. WT; †P < 0.05 vs. WTDM at the same duration of diabetes; **P < 0.05 between two groups. (A high-quality digital representation of this figure is available in the online issue.)