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. 2012 Feb 13;61(3):586–596. doi: 10.2337/db11-1091

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© 2012 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 5. — Characterization of the model-impaired insulin signaling pathway in the liver and skeletal muscle of SHHF rats. A and B: Impaired Tyr1162/3 phosphorylation of IR-β in both tissues after insulin trigger. C and D: Significantly less p85-IRS-1 attachment compared with WKY rats in both organs. E and F: Downstream effect and significantly lower Akt Ser473 phosphorylation in the obese animals in both insulin-sensitive tissues. Bar graphs show the evaluation of the phosphorylation blots relative to their corresponding loading: phospho IR-β relative to IR-β (A and B), p85 relative to IRS-1 (C and D), and phospho Akt Ser473 relative to Akt (E and F). n = 6 animals, *P < 0.05 vs. control, representative blots and immunoprecipitations of several independent experiments. IB, immunoblotting; a.u., arbitrary unit.