FIG. 6.
Hepatocyte-specific deletion of TRAF2 results in glucagon resistance. A: Plasma glucagon levels in male mice fed an HFD for 18 weeks. B: HKO and control (TRAF2flox/flox: n = 10; albumin-Cre: n = 3) males were fed an HFD for 17 weeks. Mice were fasted for 5 h and intraperitoneally injected with glucagon (10 μg/kg body wt). Blood glucose was monitored after injection and AUC was calculated. C: HKO and control male mice were fed an HFD for 18 weeks. Mice were fasted for 3 h and injected with glucagon (30 μg/kg body wt) via inferior vena. Liver nuclear extracts were prepared for 5 min after glucagon injection and immunoblotted with anti–phospho-CREB (pSer133) or anti-CREB antibodies. D: CREB phosphorylation was quantified using Odyssey software and normalized to total CREB levels. Data are mean ± SEM. *P < 0.05. Con, control; AU, arbitrary unit.