FIG. 5.

FFAs did not prevent glucose-mediated induction of islet cyclin D2 protein in vivo. A and B: Immunoblot of islets isolated after 4-day infusion showed that cyclin D2 protein was increased by hyperglycemia with or without elevated FFAs; data are quantified (B); n = 5–6. One point was excluded from the LIP group on the basis of abnormally high blood glucose (BG) in this mouse. C: A positive correlation was observed between islet cyclin D2 expression by immunoblot and average blood glucose during the preceding 4-day infusion, both in the absence (SAL and GLU; open symbols) and presence (LIP and L+G; shaded symbols) of FFAs. D: Islet cyclin D2 mRNA was not induced by glucose infusion, and there was no effect of coinfusion of lipids, although cyclin D2 mRNA levels were significantly lower in islets isolated from mice infused with lipid alone (n = 3–4). E: Immunostaining of pancreas sections after infusion showed that coinfusion of lipid did not prevent nuclear accumulation of cyclin D2 induced by glucose. Data are mean ± SEM; P values by ANOVA. ns, nonsignificant. (A high-quality digital representation of this figure is available in the online issue.)