Table 2.
Strains used in this work.
| Relevant genotype; name used in text | Source | |
|---|---|---|
| STRAIN | ||
| JG274 | MR-1, wild type | Myers and Nealson (1988) |
| JG168 | JG274 with empty pBBR1MCS-2, Kmr | Hau et al. (2008) |
| JG700 | ΔmtrB | This work |
| JG1176 | ΔmtrC/ΔomcA/ΔmtrF/ΔmtrA/ΔmtrD/ΔdmsE/ΔSO4360/ΔcctA | This work |
| JG1194 (ΔMtr) | ΔmtrC/ΔomcA/ΔmtrF/ΔmtrA/ΔmtrD/ΔdmsE/ΔSO4360/ΔcctA/ΔrecA | This work |
| JG1419 | ΔmtrB/ΔmtrC/ΔomcA/ΔmtrF/ΔmtrA/ΔmtrD/ΔdmsE/ΔSO4360/ΔcctA/ | This work |
| JG1452 | ΔmtrE/ΔmtrC/ΔomcA/ΔmtrF/ΔmtrA/ΔmtrD/ΔdmsE/ΔSO4360/ΔcctA/ΔrecA | This work |
| JG1453 | ΔmtrB/ΔmtrE/ΔmtrC/ΔomcA/ΔmtrF/ΔmtrA/ΔmtrD/ΔdmsE/ΔSO4360/ΔcctA/ | This work |
| JG1485 (ΔMtr/ΔmtrE) | ΔmtrE/ΔmtrC/ΔomcA/ΔmtrF/ΔmtrA/ΔmtrD/ΔdmsE/ΔSO4360/ΔcctA/ΔrecA | This work |
| JG1486 (ΔMtr/ΔmtrB/ΔmtrE) | ΔmtrB/ΔmtrE/ΔmtrC/ΔomcA/ΔmtrF/ΔmtrA/ΔmtrD/ΔdmsE/ΔSO4360/ΔcctA/ΔrecA | This work |
| JG1519 (ΔMtr/ΔmtrB) | ΔmtrB/ΔmtrC/ΔomcA/ΔmtrF/ΔmtrA/ΔmtrD/ΔdmsE/ΔSO4360/ΔcctA/ΔrecA | This work |
| JG1219 | JG700 with pmtrE, Kmr | This work |
| JG1220 | JG700 with pmtrB, Kmr | This work |
| JG1221 | JG700 with pdmsF, Kmr | This work |
| JG1222 | JG700 with pSO4359, Kmr | This work |
| JG1223 | JG700 with pBBR1MCS-2, Kmr | This work |
| JG1280 | JG1194 with pomcA/SO4360, Kmr | This work |
| JG1281 | JG1194 with pmrF/mtrD, Kmr | This work |
| JG1282 | JG1194 with pomcA/mtrD, Kmr | This work |
| JG1283 | JG1194 with pmtrC/mtrD, Kmr | This work |
| JG1288 | JG1194 with pmtrC/dmsE, Kmr | This work |
| JG1289 | JG1194 with pmtrF/dmsE, Kmr | This work |
| JG1290 | JG1194 with pmtrF/SO4360, Kmr | This work |
| JG1291 | JG1194 with pmtrF/mtrA, Kmr | This work |
| JG1292 | JG1194 with pomcA/dmsE, Kmr | This work |
| JG1293 | JG1194 with pmtrC/SO4360, Kmr | This work |
| JG1294 | JG1194 with pomcA/mtrA, Kmr | This work |
| JG1295 | JG1194 with pmtrC/mtrA, Kmr | This work |
| JG1489 | JG1485 with pmtrC/mtrD, Kmr | This work |
| JG1490 | JG1485 with pmtrF/mtrD, Kmr | This work |
| JG1491 | JG1485 with pomcA/mtrA, Kmr | This work |
| JG1492 | JG1485 with pmtrC/mtrA, Kmr | This work |
| JG1493 | JG1485 with pmtrF/mtrA, Kmr | This work |
| JG1504 | JG1486 with pmtrC/mtrD, Kmr | This work |
| JG1505 | JG1486 with pmtrF/mtrD, Kmr | This work |
| JG1506 | JG1486 with pomcA/mtrA, Kmr | This work |
| JG1507 | JG1486 with pmtrC/mtrA, Kmr | This work |
| JG1508 | JG1486 with pmtrF/mtrA, Kmr | This work |
| JG1525 | JG1519 with pmtrB/mtrF/mtrD, Kmr | This work |
| JG1526 | JG1519 with pmtrB/mtrC/mtrD, Kmr | This work |
| JG1527 | JG1519 with pmtrB/mtrC/mtrA, Kmr | This work |
| JG1528 | JG1519 with pmtrB/omcA/mtrA, Kmr | This work |
| JG1529 | JG1519 with pmtrB/mtrF/mtrA, Kmr | This work |
| JG1530 | JG1519 with pmtrE/mtrF/mtrD, Kmr | This work |
| JG1531 | JG1519 with pmtrE/mtrC/mtrA, Kmr | This work |
| JG1532 | JG1519 with pmtrE/mtrF/mtrA, Kmr | This work |
| JG1533 | JG1519 with pmtrE/omcA/mtrA, Kmr | This work |
| JG1534 | JG1519 with pmtrE/mtrC/mtrD, Kmr | This work |
| JG1535 | JG1485 with pBBR-BB, Kmr | This work |
| JG1536 | JG1486 with pBBR-BB, Kmr | This work |
| JG1537 | JG1519 with pBBR-BB, Kmr | This work |
| PLASMIDS | ||
| pSMV3 | Deletion vector, Kmr, sacB | Saltikov and Newman (2003) |
| pBBR1MCS-2 | Broad range cloning vector, Kmr | Kovach et al. (1995) |
| pBBR-BB | Broad range BioBrick vector, Kmr | Vick et al. (2011) |
| pmtrB | mtrB and 43 bp upstream in pBBR1MCS-2, Kmr | This work |
| pmtrE | mtrE and 43 bp mtrB upstream sequence in pBBR1MCS-2, Kmr | This work |
| pdmsF | dmsF and 43 bp mtrB upstream sequence in pBBR1MCS-2, Kmr | This work |
| pSO4359 | SO4359 and 43 bp mtrB upstream sequence in pBBR1MCS-2, Kmr | This work |
| pmtrC/mtrA | mtrA and 38 bp upstream, mtrC and 53 bp upstream in pBBR-BB, Kmr | This work |
| pmtrF/mtrA | mtrA and 38 bp upstream, mtrF and 35 bp mtrC upstream sequence in pBBR-BB, Kmr | This work |
| pomcA/mtrA | mtrA and 38 bp upstream, omcA and 53 bp mtrC upstream sequence in pBBR-BB, Kmr | This work |
| pmtrC/mtrD | mtrD and 38 bp mtrA upstream sequence, mtrC and 53 bp upstream in pBBR-BB, Kmr | This work |
| pmtrF/mtrD | mtrD and 38 bp mtrA upstream sequence, mtrF and 53 bp mtrC upstream sequence in pBBR-BB, Kmr | This work |
| pomcA/mtrD | mtrD and 38 bp mtrA upstream sequence, omcA and 53 bp mtrC upstream sequence in pBBR-BB, Kmr | This work |
| pmtrC/dmsE | dmsE and 38 bp mtrA upstream sequence, mtrC and 53 bp upstream in pBBR-BB, Kmr | This work |
| pmtrF/dmsE | dmsE and 38 bp mtrA upstream sequence mtrF and 53 bp mtrC upstream sequence in pBBR-BB, Kmr | This work |
| pomcA/dmsE | dmsE and 38 bp mtrA upstream sequence, omcA and 53 bp mtrC upstream sequence in pBBR-BB, Kmr | This work |
| pmtrC/SO4360 | SO4360 and 38 bp mtrA upstream sequence, mtrC and 53 bp upstream in pBBR-BB, Kmr | This work |
| pmtrF/SO4360 | SO4360 and 38 bp mtrA upstream sequence, mtrF and 53 bp mtrC upstream sequence in pBBR-BB, Kmr | This work |
| pomcA/SO4360 | SO4360 and 38 bp mtrA upstream sequence, omcA and 53 bp mtrC upstream sequence in pBBR-BB, Kmr | This work |
| pomcA/mtrB/mtrA | mtrA and 38 bp upstream, omcA and 53 bp mtrC upstream sequence, mtrB and 43 bp upstream in pBBR-BB, Kmr | This work |
| pmtrF/mtrB/mtrA | mtrA and 38 bp upstream, mtrF and 53 bp mtrC upstream sequence, mtrB and 43 bp upstream in pBBR-BB, Kmr | This work |
| pmtrC/mtrB/mtrA | mtrA and 38 bp upstream, mtrC and 53 bp upstream, mtrB and 43 bp upstream in pBBR-BB, Kmr | This work |
| pmtrC/mtrB/mtrD | mtrD and 38 bp mtrA upstream sequence, mtrC and 53 bp upstream, mtrB and 43 bp upstream in pBBR-BB, Kmr | This work |
| pmtrF/mtrB/mtrD | mtrD and 38 bp mtrA upstream sequence, mtrF and 53 bp mtrC upstream sequence, mtrB and 43 bp upstream in pBBR-BB, Kmr | This work |
| pomcA/mtrE/mtrA | mtrA and 38 bp upstream, omcA and 53 bp mtrC upstream sequence, mtrE and 43 bp mtrB upstream sequence in pBBR-BB, Kmr | This work |
| pmtrF/mtrE/mtrA | mtrA and 38 bp upstream, mtrF and 53 bp mtrC upstream sequence, mtrE and 43 bp mtrB upstream sequence in pBBR-BB, Kmr | This work |
| pmtrC/mtrE/mtrA | mtrA and 38 bp upstream, mtrC and 53 bp upstream, mtrE and 43 bp mtrB upstream sequence in pBBR-BB, Kmr | This work |
| pmtrC/mtrE/mtrD | mtrD and 38 bp mtrA upstream sequence, mtrC and 53 bp upstream, mtrE and 43 bp mtrB upstream sequence in pBBR-BB, Kmr | This work |
| pmtrF/mtrE/mtrD | mtrD and 38 bp mtrA upstream sequence, mtrF and 53 bpmtrC upstream sequence, mtrE and 43 bp mtrB upstream sequence in pBBR-BB, Kmr | This work |