Dendrograms based on SmaI restriction pattern resolved by pulsed-field gel electrophoresis (PFGE) depicting the relationships among representative isolates of (A) E. faecalis (feline, n = 15; surface, n = 9) and (B) E. faecium (feline, n = 18) in small animal veterinary hospitals. Strain numbers, number of strains typed (n), antimicrobial resistance (AR) profiles, and corresponding hospitals are included along each PFGE lane. “*” Indicates 96.9% similarity among E. faecalis isolated from the hospital surface and cat feces. “#” Indicates 100% similarity to E. faecium isolated from surface of a cage door and a stethoscope, and 96.9% similarity to E. faecium isolated from surface of a thermometer of the corresponding hospital (KuKanich et al., 2012); AM, ampicillin; CM, chloramphenicol; TET, tetracycline; D, doxycycline; GM, gentamicin; STR, streptomycin; RIF, rifampicin; E, erythromycin; ENO, enrofloxacin; VA, vancomycin; Q/D, quinupristin/dalfopristin; NF, nitrofurantoin. Hospital surface isolates were not screened for resistance to tetracycline, chloramphenicol, linezolid, streptomycin, and rifampicin.