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. Author manuscript; available in PMC: 2012 Feb 21.
Published in final edited form as: Cell Biochem Funct. 2010 Jan;28(1):66–73. doi: 10.1002/cbf.1622

Figure 6.

Figure 6

Total IP production response was further determined for the ERP-57 mutant receptor ERP-57 (Phe299Trp), although IP production is restricted, compared to the chaperone free production, no difference was observed in the effect of this mutant compared to the non-mutant ERP-57; such an observation is also made when this mutant was co-transfected with calnexin. All Cos 7 cells were transiently transfected with hWT GnRH cDNA (25 ng) and different chaperones were added: ERP-57 (75 ng), ERP-57/Phe299Trp mutant (75ng), Calnexin (75ng), ERP-57 (50ng) and CANX (25ng), ERP-57/Phe299Trp mutant (50 ng) and CANX (25ng).