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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Nat Immunol. 2011 Aug 7;12(9):844–852. doi: 10.1038/ni.2080

Figure 1. IKKi forms a complex with Act1 upon IL-17 stimulation.

Figure 1

A. Cell lysates from Act1-deficient MEFs infected with retroviral WT Act1 (Act1-WT) untreated or treated with IL-17 (50 ng/ml) for 0, 15, 30 and 60 min were immunoprecipitated with anti-Act1 or IgG, followed by immunoblot analysis with anti-IKKi, anti-Act1 and anti-GAPDH. WCL: whole cell lysates.

B. Lysates from Act1 WT reconstituted MEFs treated with IL-17 (50 ng/ml) for 0, 15 and 30 min were untreated or treated with phosphatase (CIP, 1 h, 37°C)], followed by immunoblot analysis with anti-Act1 and anti-GAPDH.

C. Cell lysates from wild-type and Act1-deficient MEFs untreated or treated with IL-17 (50 ng/ml) for 0, 15 and 30 min were immunoprecipitated with anti-IKKi, followed by immunoblot analysis with anti-Act1 and anti-IKKi. WCL: whole cell lysates.

The data shown in this figure are representation of three independent experiments.