A. Total BAL and differential cell count were analyzed in samples from control or IL-17 challenged (1 µg through intranasal injection for 24 h) wild-type and IKKi-deficient mice (n=6) P<0.05. Cytospins prepared from the BAL of control or IL-17 challenged wild-type and IKKi-deficient mice were stained with Hema3, and differential cell counting was performed using standard morphological criteria. Magnification x400.
B. The lung sections from control or IL-17 challenged wild-type and IKKi-deficient mice (1 µg through intranasal injection for 24 h) were stained with H&E. Magnification x100.
C. ELISA of CXCL1 chemokine in BAL fluid from control or IL-17 challenged wild-type and IKKi-deficient mice.
D. Real-time PCR analysis of CXCL1, TNF, CXCL2, IL-6 and CSF3 in the lung tissues from control or IL-17 treated wild-type and IKKi-deficient mice. Expression of mRNA is presented as arbitrary units (mean ± s.d.) relative to the expression of mRNA encoding β-Actin. P<0.05.
The data shown in this figure are representation of two independent experiments.