FIGURE 4.

ROCK2 activates NF-κB by phosphorylating the p65 subunit. siRNA-mediated gene knockdowns were performed as described in the legend to Fig. 3, and cells were then exposed to LPA for 30 min. Effectiveness of gene knockdown was assessed by Western blotting with ROCK1- and ROCK2-specific antibodies (top two panels). Phospho-NF-κB p65 was measured with a phosphospecific antibody and total NF-κB p65 and GAPDH were used as normalization controls.