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. 2011 Sep 30;38(3):135–141. doi: 10.5653/cerm.2011.38.3.135

Figure 3.

Figure 3

Reverse transcriptase-polymerase chain reaction (RT-PCR) detection for leptin expression in ovaries of young (6-9 weeks old) and aged (24-26 weeks old) female mice. Whole ovaries were collected 0, 6, 12, 24, and 48 hours after pregnant mare's serum gonadotropin (PMSG) injection (n=4/point in time). The graphical representation of leptin intensity normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) are shown below a representative example of RT-PCR (n=4). ap<0.05, bp< 0.01, and cp<0.005.