Figure 5.

Effect of leptin on visfatin, endothelial nitric oxide synthase (eNOS), and vascular endothelial growth factor (VEGF) mRNA expression in cultured mouse ovaries. Ovaries from 11 weeks old female mice were placed into culture and treated with leptin (0, 0.01, 0.1, and 1 µg/mL) for 7 days. Leptin, visfatin, eNOS, and VEGF mRNA levels were detected by reverse transcriptase-polymerase chain reaction (RT- PCR). The graphical representation of the intensity of each gene normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) are shown below a representative example of RT-PCR (n=3). ^ap<0.005 (vs. 0 µg/mL).