Skip to main content
. 2011 Dec;59(12):1060–1075. doi: 10.1369/0022155411428078

Figure 7.

Figure 7.

(A) CD146/MART-1 co-labeling by immunohistochemistry (IHC) of tumor sections derived from B16-induced melanomas. In this image, an intratumoral vessel constituted by perivascular CD146+ cells (left and right panels, in green and pointed by white arrows) and vessel-lining MART-1+ cells (center and right panels, in red and pointed by arrowheads) is observed. (B) CD31/MART-1 co-labeling by IHC of tumor sections derived from B16-induced melanomas. In this image, an intratumoral blood vessel composed of MART-1+ blood vessel–lining cells (center and right panels, in red) lacking CD31 expression (left and right panels) is observed. (C) HIF-1α/MART-1 co-labeling by IHC of tumor sections derived from B16-induced melanomas. Numerous cells within the tumor are observed to express HIF-1α (left and right panels, in green) in the nucleus (in blue). HIF-1α-expressing cells comprise both MART-1+ cancer cells (central and right panels, in red) and MART-1− stromal cells. HIF-1α expression also localizes to blood vessel–lining MART-1+ cells associated with intratumoral vessels. Asterisks are located in the center of intratumoral blood vessels. Scale bars: 50 µm.