Table 1.
Putative Stromal Stem Cell Subpopulations Identified in the MART-1− Stroma Fraction of the Tumor and Surrounding Skin and Muscle of 7- and 14-Day-Old B16-induced Melanomas
| Stromal Subpopulations in Tumor and Adjacent Skin and Muscle of B16-induced Melanomas |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD45–, CD146– |
CD45–, CD146+ |
CD45–, CD146– |
CD45–, CD146– |
CD45–, CD146+ |
CD45–, CD146+ |
||||||||
| c-Kit–, Sca-1+ |
c-Kit–, Sca-1− |
c-Kit+, Sca-1− |
c-Kit+, Sca-1+ |
c-Kit–, Sca-1+ |
c-Kit+, Sca-1− |
||||||||
| Mean, % | SD | Mean, % | SD | Mean, % | SD | Mean, % | SD | Mean, % | SD | Mean, % | SD | ||
| Tumor | 7 days | 15.6 | 0.07 | 31.1 | 2.6 | 1.4 | 0.98 | 0.1 | 0.03 | 1.43 | 0.65 | 0 | 0 |
| 14 days | 2.8 | 1.92 | 35.3 | 7.74 | 0.6 | 0.36 | 0.25 | 0.14 | 0.3 | 0.14 | 1.8 | 0.62 | |
| Skin | Control | 9.65 | 0.84 | 1.6 | 0.84 | 0 | 0 | 0.7 | 0.14 | 1.35 | 0.35 | 0.65 | 0.21 |
| 7 days | 51.25 | 12.05 | 0.35 | 0.5 | 0 | 0 | 0.1 | 0.14 | 0.35 | 0.21 | 1 | 0.42 | |
| 14 days | 13.95 | 3.04 | 7.3 | 1.55 | 0.6 | 0.84 | 0.05 | 0.07 | — | — | 0.15 | 0.07 | |
| Muscle | Control | 1.4 | 0.42 | 1.1 | 0.56 | 0.05 | 0.07 | 0.05 | 0.07 | 0.65 | 0.21 | 0.05 | 0.07 |
| 7 days | 28.8 | 0.7 | 1.8 | 2.54 | 0 | 0 | 0.55 | 0.35 | 0 | 0 | 0 | 0 | |
| 14 days | 14.3 | 13.53 | 49 | 9.98 | 0.8 | 0.28 | 0 | 0 | — | — | 3.8 | 0.98 | |
For 7-day old tumor analysis, a total of four tumors were analyzed. For 14-day tumor analysis, a total of six tumors were analyzed. The data were obtained by flow cytometry, and the results are expressed as the mean value ± SD. Statistical significance was ascertained with a Dunnett posttest; **p<0.001, ***p<0.0001 with respect to the control.