The series of pictures show immunohistochemical staining results of the same cylinder at baseline (A) and after 12 months of storage at RT (B), at RT with a coating of paraffin (C), at 4C (D), and at 4C with paraffin coating (E). The graph shows the overall mean gray value for the different storing times at different storing conditions. Diaminobenzidine was used as chromogen showing brown color. 4 = 4C, pf = paraffin-coating, RT = room temperature. A-E, bar 100 µm.