Table 1.
Genes Surveyed in this Study.
| Gene | Type | Function/Effects | Max. Tissue | τ | Sex | Sample Size D. melanogaster | Sample Size D. simulans |
| CG8982 (Acp26Aa, ovulin) | Target | Ovulation | Male acc. gland | 1 | M | 13 | 14 |
| CG7157 (Acp36DE) | Target | Sperm storage | Male acc. gland | 1 | M | 11 | 10 |
| CG17673 (Acp70A, sexpeptide) | Target | Remating, eggproduction and laying, feeding | Male acc. gland | 1 | M | 14 | 12 |
| CG1262 (Acp62F) | PI | Sperm competition, toxic | Male acc. gland | 1 | M | 16 | 13 |
| CG1342 | PI | Unknown | Male acc. gland | NA | M | 14 | 11 |
| CG8137 | PI | Toxic | Male acc. gland | 1 | M | 11 | 14 |
| CG10956 | PI | Unknown | Male acc. gland | 1 | M | 14 | 8 |
| CG31902 | PI | Unknown | Male acc. gland | 1 | M | 12 | 11 |
| CG32203 | PI | Unknown | Male acc. gland | 1 | M | 14 | 13 |
| CG33121 | PI | Unknown | Male acc. gland | 1 | M | 14 | 14 |
| CG9997 | Prot. hom. | Remating, eggproduction and laying, sperm release | Male acc. gland | 1 | M | 13 | 12 |
| CG11864 | Prot. | Cleavage of ovulin | Male acc. gland | 1 | M | 12 | 15 |
| CG6168 | Prot. | Immunity | Male acc. gland | 1 | M | 18 | 12 |
| CG32382 | Prot. hom. | Immunity | Male acc. gland | 1 | M | 14 | 11 |
| CG32383 | Prot. hom. | Immunity | Male acc. gland | 1 | M | 14 | 8 |
| CG1895 | Prot. | Unknown | Male acc. gland | 1 | M | 15 | 14 |
| CG6069 | Prot. hom. | Unknown | Male acc. gland | 1 | M | 20 | 12 |
| CG9806 | Prot. | Unknown | Male acc. gland | 0.99 | M | 14 | 11 |
| CG10586 | Prot. | Unknown | Male acc. gland | 1 | M | 14 | 15 |
| CG10587 | Prot. | Unknown | Male acc. gland | 1 | M | 16 | 13 |
| CG11037 | Prot. | Unknown | Male acc. gland | 1 | M | 16 | 15 |
| CG11664 | Prot. hom. | Unknown | Male acc. gland | 1 | M | 14 | 14 |
| C13518 | Prot. | Unknown | Male acc. gland | NA | M | 14 | 13 |
| CG17242 | Prot. | Unknown | Male acc. gland | 1 | M | 13 | 12 |
| CG18557 | Prot. | Unknown | Male acc. gland | 0.85 | M | 14 | 13 |
| CG4847 | Prot. | Unknown | Male acc. gland | 0.96 | M | 10 | 9 |
| CG32833 | Prot. | Unknown | Male acc. gland | 1 | M | 14 | 12 |
| CG9456 | PI | Unknown | Male acc. gland | 0.96 | F + M | 14 | 14 |
| CG10363 (TepIV) | PI | Immunity | Hindgut | 0.77 | F + M | 13 | 15 |
| CG1857 (necrotic) | PI | Immunity | Fat body | 0.8 | F | 12 | 12 |
| CG11331 | PI | Immunity | Crop | 0.86 | F | 15 | 14 |
| CG1865 | PI | Unknown | Spermathecae | 0.7 | F | 12 | 13 |
| CG3604 | PI | Unknown | Hindgut | 0.99 | F | 16 | 15 |
| CG18525 | PI | Unknown | Spermathecae | 0.8 | F | 15 | 14 |
| CG3066 | Prot. | Immunity | Crop | 0.68 | F | 14 | 12 |
| CG3074 | Prot. | Eggshell matrix | Crop | 0.8 | F | 14 | 12 |
| CG3097 | Prot. | Unknown | Crop | 0.91 | F | 15 | 13 |
| CG9849 | Prot. | Unknown | Salivary gland | 0.5 | F | 13 | 12 |
| CG9897 | Prot. hom. | Unknown | Spermathecae | 1 | F | 7 | 13 |
| CG13318 | Prot. hom. | Unknown | Spermathecae | 0.95 | F | 15 | 12 |
| CG14642 | Prot. | Unknown | Spermathecae | 0.86 | F | 11 | 12 |
| CG18125 | Prot. | Unknown | Spermathecae | 1 | F | 12 | 12 |
| CG31199 | Prot. | Unknown | Eye | 0.98 | F | 13 | 12 |
| CG31681 | Prot. | Unknown | Spermathecae | 1 | F | 14 | 14 |
Note. —Targets are proteins known to undergo proteolysis following mating. PI: Predicted protease inhibitors. Prot.: Predicted catalytic proteases. Prot. hom.: Predicted protease homologs. “Max. tissue” indicates tissue of highest expression, and τ is the tissue specificity measure of Yanai et al. (2005), with τ = 1 indicating absolute specificity and τ = 0 indicating equal expression in all tissues. “NA” in the τ column indicates that τ cannot be calculated because of low expression levels. “Sex” indicates whether a gene was included because of its identification in screens of the male (M) or female (F) reproductive tracts (Swanson et al. 2001; Swanson et al. 2004).