Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 15;139(6):1133–1140. doi: 10.1242/dev.073478

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012.

PMC Copyright notice

Fig. 5. — Ectopic accumulation of DNA repair factors in Brg1^CKO spermatocytes. (A-D) Distribution of repair proteins RAD51 (green; A,B) and RPA (green; C,D) in wild-type (A,C) and Brg1^CKO (B,D) spermatocytes. SYCP3 (red) was used in nuclear spreads to identify spermatocytes in the zygotene or pachytene stages. Arrowheads in B and D depict abnormal repair foci of Brg1^CKO. (E,F) Crossover formation in pachytene spermatocytes. MLH1-containing recombination nodules (green) on the synaptic axes (red) in wild-type (E) and mutant spermatocytes (F) are shown in nuclei spreads prepared from 15 dpp testis. Numbers in representative images (E,F) indicate the number of MLH1 foci per nucleus. (G) Beeswarm plot of MLH1 foci in each control (black circle, n=110) and Brg1^CKO (open circle, n=108) spermatocyte. The red line is the average number of MLH1 foci in each genotype (21.1 in wild type versus 17.3 in Brg1^CKO; P=2.3x10^–6); statistical significance was determined by t-test. Pa, pachynema; Zy, zygonema.