Fig. 2.

sdp alleles exhibit lesions in the reck coding sequence. (A) Physical map of the end of chromosome 24. sdp critical region (red line) contained at least four genes; simple sequence length polymorphism (SSLP) markers used for mapping are shown and locations of lesions indicated. (B) Predicted protein product zebrafish reck is 956 amino acids long and has an N-terminal signal sequence and two Kazal motifs. (C) Electropherograms showing each of the reck single nucleotide polymorphisms (SNPs). sdp^w12 is a splice site mutation at the exon 9-intron 9 boundary, sdp^w13 is a C38R substitution in exon 2, sdp^w14 is a W252X substitution in exon 9. (C′) sdp^w15 is a large deletion of chromosome 24 encompassing at least 0.4 Mb and including the entire sdp critical region.