Table 1. Primers used for PCR.
Exon | Forward (5′-3′) | Reverse (5′-3′) | Product size (bp) | Annealing temperature (°C) |
---|---|---|---|---|
FGFR2-8 (IIIa) |
GGTCTCTCATTCTCCCATCCC |
CCAACAGGAAATCAAAGAACC |
325 |
61 |
FGFR2-10 (IIIc) | CCTCCACAATCATTCCTGTGTC | ATAGCAGTCAACCAAGAAAAGGG | 257 | 61 |
Summary of the primers and products length used for the amplification of the exons of FGFR 2.