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. 2011 Dec 1;287(5):3123–3137. doi: 10.1074/jbc.M111.324350

FIGURE 7.

FIGURE 7.

Knockdown of NEP1-R1 and lipin-1 in C2C12 cells has similar effects on expression of dystrophin and Serca2. Top row, gene expression changes in muscle from lipin-1-deficient fld mice. Lack of lipin-1 in muscle leads to enhanced expression of dystrophin and reduced expression of Serca2. Middle row, knockdown of lipin-1 expression in C2C12 myocytes using a lipin-1-specific antisense oligonucleotide (ASO) recapitulates the effects on dystrophin and Serca2 gene expression observed in fld muscle and leads to reduced expression of NEP1-R1. Bottom row, knockdown of NEP1-R1 expression in C2C12 myocytes using a specific siRNA leads to similar changes in dystrophin and Serca2 expression as observed in fld muscle. NEP1-R1 knockdown does not affect lipin-1 mRNA levels. Relative mRNA levels represent the mean ± S.D. for three separate cultures. *, p < 0.05 or **, p < 0.01 versus relevant wild type or control sample.