FIGURE 3.

Mapping of interacting domains between Gas7 and N-WASP. A, schematic representation of various Gas7 (upper panel) and N-WASP truncates (lower panel) according to functional domains. Open triangles and diamonds indicate GST and His tags, respectively. B, Gas7 derivatives (A, upper panel) were produced in vitro, purified, and used in a pulldown assay with purified His-tagged N-WASP. His-N-WASP was pulled down by WW domain-containing proteins (GST-Gas7, GST-Gas7WW1, and GST-Gas7WW2). C, His-N-WASP derivatives (A, lower panel) were generated in vitro, purified, and used in a pulldown assay with immobilized Gas7. The Pro region of N-WASP was pulled down by GST-Gas7 but not by GST (negative control). WW, WW domain; WH1, WASP homology domain 1; Pro, proline-rich region.